Learn More About IHR’s testing options
Diagnostic Testing Options
Fertility Treatment that’s Optimized for Your Success
Diagnostic Tests That May be Included in Your Treatment
The tests featured below may help provide infertility answers or create a more detailed treatment plan for you to reach your family-building goals.
The ERA test evaluates the status of the woman’s endometrial receptivity to prevent implantation failure. The goal of this test is to find a personalized window of Implantation to allow for a personalized embryo transfer (pET), optimizing the best time to transfer the embryo and therefore increasing the chance of conception.
The ERA test uses a small sample of endometrial tissue to find the best day for transferring the embryo. The biopsy is easily and quickly sampled, and the results will determine if the endometrium is receptive or not at the time of sampling. The ERA test analyzes the expression levels of 248 genes linked to the status of endometrial receptivity using RNA sequencing taken from the endometrial tissue.
When performing the embryo transfer in a personalized way, data shows an increased likelihood of a successful conception. Interim data analysis of a prospective randomized trial, presented in 2016, demonstrated a significant 24% increase in pregnancy rate with pET. In other studies, the ERA test has resulted in a 73% pregnancy rate in patients with implantation failure.
For many years, infertility specialists waited to investigate ‘implantation failure’ until after two to three failed embryo transfers. Because the lack of synchronization between the embryo ready to be implanted and endometrial receptivity is one of the causes of recurrent implantation failure, it’s imperative to assess the endometrium and determine the optimal day for embryo transfer.
What is a semen analysis?
A semen analysis is recommended for couples who are having difficulty conceiving and/or with recurrent miscarriage. The sample is evaluated for the number of sperm, their motility and their shape. Abnormalities in any of these can affect reproductive health. Because sperm are continuously being produced, abnormal samples may require re-testing to confirm findings.
This test requires an actual appointment at the Chicago office location.
The following instructions are recommended
- A semen specimen is best produced by masturbation following a two to five day period of sexual abstinence.
- Avoid soaps, detergents, creams or lubricants to aid in specimen collection. These agents can cause damage to the sperm.
- Collect the entire specimen in the sterile container. If there is any spillage, let the lab know if it occurred toward the beginning or end of the specimen collection.
- Most importantly, please relax! If there are any problems with the collection process, please let us know.
If you require to produce a sperm sample at an outside facility
- an appointment is still required to ensure lab staff will be available to process the specimen.
- You will need to obtain a sterile container and materials, from our office, prior to your appointment.
- On day of appointment, the specimen should be delivered to our office within an hour of collection. Keep the specimen as close to body temperature as possible during transportation.
- Do not expose the specimen to hot or cold extremes.
A hysterosalpingogram is a diagnostic x-ray test where dye is injected into the uterus and fallopian tubes. The test is usually scheduled between cycle days 5-11. If you are on birth control pills, this test may be completed any time. The hysterosalpingogram can evaluate the uterine cavity, the patency of the fallopian tubes and positioning of the uterus in relation to the tubes. This test is performed at the 900 N. Surgical Center. At this appointment, you will also have a physical exam if not already completed at a previous appointment. A hysterosalpingogram or HSG is an x-ray procedure used to see whether the fallopian tubes are patent (open) and if the inside of the uterus (uterine cavity) is normal. HSG is an outpatient procedure that usually takes less than 5 minutes to perform. It is usually done after the menstrual period ends but before ovulation.
How is a hysterosalpingogram done?
A woman is positioned under a fluoroscope (a x-ray imager that can take pictures during the study) on a table. The doctor examines the patient’s uterus and places a speculum in her vagina. Her cervix is cleaned, and a device (cannula) is placed into the opening of the cervix. The doctor gently fills the uterus with a liquid containing iodine (a fluid that can be seen by x-ray) through the cannula. The contrast will be seen as white on the image and can show the contour of the uterus as the liquid travels from the cannula, into the uterus, and through the fallopian tubes. As the contrast enters the tubes, it outlines the length of the tubes and spills out their ends if they are open.
Abnormalities inside the uterine cavity may also be detected by the doctor observing the x-ray images when the fluid movement is disrupted by the abnormality. The HSG procedure is not designed to evaluate the ovaries or to diagnose endometriosis, nor can it identify fibroids that are outside of the endometrial cavity, either in the muscular part of the uterus, or on the outside of the uterus. Often, side views of the uterus and tubes are obtained by having the woman change her position on the table. After the HSG, a woman can immediately return to normal activities, although some doctors ask that she refrain from intercourse for a few days.
Is it uncomfortable?
An HSG usually causes mild or moderate uterine cramping for about 5-10 minutes. However, some women may experience cramps for several hours. These symptoms can be greatly reduced by taking medications used for menstrual cramps before the procedure or when they occur. Women should be prepared to have a family member or friend drive them home after the procedure in the event that they are experiencing cramping.
Does a hysterosalpingogram enchance fertility?
It is controversial whether this procedure enhances fertility. Some studies show a slight increase in fertility lasting about 3 months after a normal HSG. However, most doctors perform HSG only for diagnostic reasons.
What is the next step if my tubes are blocked?
- If your tubes are blocked, your doctor will likely recommend either a surgical procedure to directly view the tubes (laparoscopy) or to bypass the tubes and perform in vitro fertilization (IVF). This is a complex decision that should be discussed with your doctor. For more information, please see the ASRM booklet Laparoscopy and hysteroscopy and fact sheet What do I need to know about conceiving after tubal surgery?
- Are there other options to evaluate tubal patency?
- Laparoscopy can also determine if tubes are open, using a procedure called chromopertubation. An alternative procedure to evaluate tubal patency is a sonohysterosalpingogram (SHG). For SHG, a catheter (narrow tube) is placed in the uterus through the vagina and saline and air are injected. In women who have open fallopian tubes, tiny air bubbles may be seen going through the fallopian tubes during the ultrasound. However, this procedure is inferior to HSG for assessment of tubal patency.
This is a surgical procedure in which a lighted telescope-like instrument (laparoscope) is inserted through the wall of the abdomen into the pelvic cavity. Laparoscopy is useful to evaluate the pelvic cavity for endometriosis, pelvic adhesions, and other abnormalities.
Laparoscopy is not a first line option in the evaluation of a female patient. Because of its higher costs and potential surgical risk, it may be recommended depending on the results of other testing and the woman’s history, such as pelvic pain and previous surgeries.
What is a Hysteroscopy?
This is a surgical procedure in which a lighted telescope-like instrument (hysteroscope) is passed through the cervix to view the inside of the uterus.
Hysteroscopy can help diagnose and treat abnormalities inside the uterine cavity such as polyps, fibroids and adhesions (scar tissue). These abnormalities can prevent pregnancy or contribute to the cause of miscarriage.
This procedure is known by a few names: Hysterogsonogram (HSN) or sonohysterography (SHG) is a procedure to evaluate the uterus and the shape of the uterine cavity. SHG uses ultrasound and sterile fluid to show the uterus and endometrial (uterine lining) cavity. The ovaries are also seen at the time of SHG. The purpose is to detect any abnormalities. The HSN provides the best imaging of the contour of the uterine cavity. It is an ultrasound where fluid is injected into the uterus. The test is usually scheduled between cycle days 5-11. If you are on birth control pills, this test may be completed any time. The HSN evaluates the uterine cavity and can also, sometimes, check the patency of the fallopian tubes. This test is performed at our Chicago office location. At this appointment, you will also have a physical exam if not already completed at a previous appointment.
Why is SHG performed?
- HSN can be done to investigate conditions such as abnormal uterine bleeding, infertility, and recurrent miscarriage. HSN can also be performed to see the structure of the uterus.
- This may be done in women with congenital abnormalities (birth defects) of the uterus, before and after surgery on the uterus, or to detect problems that appear later in life such as polyps or suspected scar tissue inside the uterus.
- HSN may also help check uterine abnormalities found during a routine ultrasound.
- HSN should not be performed in women who are pregnant or who are suspected to be pregnant. SHG should also not be performed in women with an active pelvic infection.
How is HSN performed?
- HSN is usually done after the menstrual period finishes. In women not menstruating (such as those on medications suppressing the menstrual cycle, post- menopausal women, etc.), it may be done at any time.
- The procedure begins with an ultrasound examination using a probe placed in the vagina.
- Next, a speculum is introduced and a narrow catheter is placed in the vagina, through the cervix, and into the uterine cavity. The ultrasound examination is continued while sterile saline (salt water) is put into the uterus.
- The saline solution fills the uterus, helping to outline the uterine walls and cavity. This shows abnormalities such as fibroids, polyps, or scar tissue inside the uterus
What are the risks and complications?
- HSN is a very safe procedure and usually is performed without incident. Serious complications are rare. The most common serious complication with SHG is pelvic infection. However, this occurs less than 1% of the time and usually occurs when a woman also has a block or infection of the fallopian tubes.
- HSN may also cause cramping, spotting, and vaginal discharge. Some women have cramping for several hours after the procedure. It is often recommended to take a medication such as ibuprofen before this test. Some doctors may also prescribe stronger pain medication and/or antibiotics before the procedure. You should call your doctor if you experience pain or fever in the 1–2 days after the SHG.
Your physician will determine the best test(s)for you based on your medical history. Some patients who completed an HSG will also need an HSN based on the results of the HSG. Small irregularities that may not be identified by the HSG can be detected by this evaluation. By the same token, some patients who completed an HSN will also require an HSG if there are unanswered questions regarding the fallopian tubes. As you complete either test, your physician will share the results with you and let you know if more testing is recommended.